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What are the fluorescence optic detection modules?
The Integrated Cycler Studio Software has four fluorescence optic detection modules including, 520 nm (FAM or equivalent), 560 nm (JOE or equivalent), 610 nm (Cal Fluor Red or equivalent), 682 nm (Quasar 670 or equivalent).
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Is routine in-field calibration necessary for the 3M™ Integrated Cycler?
No. The instrument is calibrated at the factory.
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Can a new assay be defined or new run created while the instrument is in use?
Yes. Assays can be defined and edited, and new runs setup while the instrument is in use. The assays will be saved until the instrument has completed its current run.
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Can assay definitions be readily transferred to another instrument?
Yes. User-defined assay definitions can be exported and imported to instruments using the Manage Assays administrative functions.
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Can Thresholds be manually adjusted when using the Integrated Cycler Studio Software for user-defined purposes?
Yes. The Thresholds can be adjusted while analyzing data for user-defined assay definitions.
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Why didn’t the positive controls amplify?
PCR enzymes are heat labile. Store reagents as described in the product literature. Avoid freeze thaw cycles.
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Why were results inconsistent?
Decontaminate the work area, use fresh reagents, avoid cross-contaminating samples, and check sample order entered on Integrated Cycler Studio Software.
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How can I login to the Integrated Cycler Studio Software?
Contact the Lab Manager or Administrator.
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Why is the instrument not responding to software commands, and/or the front panel LED is solid or blinking green?
The instrument has lost connection with the Integrated Cycler Studio Software. Verify connection or select FIND INSTRUMENT under the file menu.
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Where is the error messages explained?
Error messages are explained in the Integrated Cycler Studio Software Operator Manual.